Hotspot

rps4

LSC · 45239-45844 · 606 bp

Back to Xylosalsola

Pi 0.00165

MarkerSeek score 59.70

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: LSC
length_bp: 606
variable_sites: 1
pi: 0.00165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	AGAAAGGGGCCAAATTGTCC	TCGTTTCATTTACCATTTCCA	50.0	33.3	58.4	53.7	775 bp	1.0	40.1
rps4_p2	ACTTTGGCGAAAGAAAGGGG	TCGTTTCATTTACCATTTCCA	50.0	33.3	58.7	53.7	786 bp	1.0	40.1
rps4_p3	AGAAAGGGGCCAAATTGTCC	TCGTTTCATTTACCATTTCCAT	50.0	31.8	58.4	54.3	775 bp	1.0	40.1
rps4_p4	AGAAAGGGGCCAAATTGTCC	TCGTTTCATTTACCATTTCCATT	50.0	30.4	58.4	55.1	775 bp	1.0	40.1
rps4_p5	CCAAATTGTCCGAACCCTTTG	TCGTTTCATTTACCATTTCCA	47.6	33.3	58.0	53.7	766 bp	1.0	40.1