Hotspot

rps3

LSC · 82707-83363 · 657 bp

Back to Xylosalsola

Pi 0.00152

MarkerSeek score 59.60

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 657
variable_sites: 1
pi: 0.00152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TGGATGGAAAAAGGGATGTCA	TGTCTGGACGGTTTTTGGAA	42.9	45.0	57.2	57.6	816 bp	1.0	62.1
rps3_p2	TGGATGGAAAAAGGGATGTCA	TTGTCTGGACGGTTTTTGGA	42.9	45.0	57.2	57.6	817 bp	1.0	62.1
rps3_p3	TTGGATGGAAAAAGGGATGTCA	TGTCTGGACGGTTTTTGGAA	40.9	45.0	57.9	57.6	817 bp	1.0	59.7
rps3_p4	TTGGATGGAAAAAGGGATGTCA	TTGTCTGGACGGTTTTTGGA	40.9	45.0	57.9	57.6	818 bp	1.0	59.7
rps3_p5	TGGATGGAAAAAGGGATGTCAT	TGTCTGGACGGTTTTTGGAA	40.9	45.0	57.7	57.6	816 bp	1.0	58.0