Hotspot

trnN-GUU-trnR-ACG

IRa · 128204-128808 · 605 bp

Back to Xylosalsola

Pi 0.00165

MarkerSeek score 59.70

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 605
variable_sites: 1
pi: 0.00165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGGTTGAGAACGGGAATTG	GGATACTCCCGGGAACAGAT	50.0	55.0	58.1	58.3	836 bp	1.0	77.1
trnN-GUU-trnR-ACG_p2	TGGTCGTAGGTTCGAATCCT	GGATACTCCCGGGAACAGAT	50.0	55.0	58.2	58.3	746 bp	1.0	76.6
trnN-GUU-trnR-ACG_p3	TGGGTTGAGAACGGGAATTG	CAGTGGATTAGAGCACGTGG	50.0	55.0	58.1	58.4	787 bp	1.0	76.5
trnN-GUU-trnR-ACG_p4	GGGTTGAGAACGGGAATTGA	GGATACTCCCGGGAACAGAT	50.0	55.0	57.8	58.3	835 bp	1.0	76.3
trnN-GUU-trnR-ACG_p5	TGGTCGTAGGTTCGAATCCT	CAGTGGATTAGAGCACGTGG	50.0	55.0	58.2	58.4	697 bp	1.0	76.0