Hotspot

trnN-GUU-ndhF

IRb · 107321-108060 · 740 bp

Back to Xylosalsola

Pi 0.0191

MarkerSeek score 66.10

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 740
variable_sites: 14
pi: 0.0191
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGGATTCGAACCTACGACCA	GGGGGTCGAATCTCCTCTTA	50.0	55.0	58.2	57.9	883-2347 bp	1.0	79.1
trnN-GUU-ndhF_p2	AGGATTCGAACCTACGACCA	ATCAAGTATGGAGGAGGGGG	50.0	55.0	58.2	57.9	898-2362 bp	1.0	78.8
trnN-GUU-ndhF_p3	AATCAGTTAACAGCCGACCG	GGGGGTCGAATCTCCTCTTA	50.0	55.0	58.3	57.9	864-2328 bp	1.0	78.2
trnN-GUU-ndhF_p4	AATCAGTTAACAGCCGACCG	ATCAAGTATGGAGGAGGGGG	50.0	55.0	58.3	57.9	879-2343 bp	1.0	77.8
trnN-GUU-ndhF_p5	CCCCAAGTAGGATTCGAACC	GGGGGTCGAATCTCCTCTTA	55.0	55.0	57.7	57.9	891-2355 bp	1.0	77.7