Hotspot

trnP-UGG-psaJ

LSC · 66620-67051 · 432 bp

Back to Xylosalsola

Pi 0.00481

MarkerSeek score 59.60

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 432
variable_sites: 2
pi: 0.00481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	TGTCAACGCATCTGGGAAAA	50.0	45.0	58.1	58.0	585-601 bp	1.0	81.4
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	TGTCAACGCATCTGGGAAAA	50.0	45.0	58.1	58.0	584-600 bp	1.0	81.4
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	TGTCAACGCATCTGGGAAAA	50.0	45.0	58.1	58.0	596-612 bp	1.0	81.3
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	TGTCAACGCATCTGGGAAAA	50.0	45.0	58.1	58.0	595-611 bp	1.0	81.3
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	TGTCAACGCATCTGGGAAAA	50.0	45.0	58.1	58.0	598-614 bp	1.0	81.3