Hotspot

psbE-petL

LSC · 64635-65779 · 1145 bp

Back to Xylosalsola

Pi 0.00262

MarkerSeek score 60.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1145
variable_sites: 3
pi: 0.00262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCGGAGGTAATAGTTAAAGCC	50.0	45.5	57.9	57.9	1309-1312 bp	1.0	63.2
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GGAGGTAATAGTTAAAGCCGCT	50.0	45.5	57.9	57.9	1306-1309 bp	1.0	63.2
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AGCGGAGGTAATAGTTAAAGCC	50.0	45.5	57.8	57.9	1232-1235 bp	1.0	62.4
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	GGAGGTAATAGTTAAAGCCGCT	50.0	45.5	57.8	57.9	1229-1232 bp	1.0	62.4
psbE-petL_p5	ACGAATAACCAACCCGCAAT	GGAGGTAATAGTTAAAGCCGCT	45.0	45.5	58.2	57.9	1315-1318 bp	1.0	62.3