Hotspot

accD-psaI

LSC · 58797-59473 · 677 bp

Back to Xylosalsola

Pi 0

MarkerSeek score 60.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 677
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGGACCTAATCGTACCACGT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	830-839 bp	1.0	79.4
accD-psaI_p2	TGGACCTAATCGTACCACGT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.2	57.9	836-845 bp	1.0	78.7
accD-psaI_p3	TTGGACCTAATCGTACCACG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.1	58.0	831-840 bp	1.0	73.1
accD-psaI_p4	TGGACCTAATCGTACCACGTA	CCATTGCAATTGCCGGAAAT	47.6	45.0	58.0	58.0	830-839 bp	1.0	72.6
accD-psaI_p5	TGGACCTAATCGTACCACGT	CCGGAAATACTAGGCCCACT	50.0	55.0	58.2	58.9	818-827 bp	1.0	72.6