Hotspot

psbK-psbI

LSC · 7075-7495 · 421 bp

Back to Xylosalsola

Pi 0.00238

MarkerSeek score 56.40

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 421
variable_sites: 1
pi: 0.00238
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GCCAAATTACCGGAAGCCTA	TTTTATTCTTCGCGCCCAGG	50.0	50.0	58.0	58.9	651 bp	1.0	72.5
psbK-psbI_p2	GCCAAATTACCGGAAGCCTA	TTTTTATTCTTCGCGCCCAG	50.0	45.0	58.0	57.1	652 bp	1.0	72.5
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	TTTTATTCTTCGCGCCCAGG	50.0	50.0	57.9	58.9	564 bp	1.0	72.4
psbK-psbI_p4	GTTTGGCAAGCTGCTGTAAG	TTTTTATTCTTCGCGCCCAG	50.0	45.0	57.9	57.1	565 bp	1.0	72.4
psbK-psbI_p5	TGGCAAGCTGCTGTAAGTTT	TTTTATTCTTCGCGCCCAGG	45.0	50.0	58.3	58.9	561 bp	1.0	70.4