Hotspot

matK-trnK-UUU

LSC · 3442-4162 · 721 bp

Back to Xylosalsola

Pi 0.00139
MarkerSeek score 60.10
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
721
variable_sites
1
pi
0.00139
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 TCAAGTTATGTTGCCAAGACCT AACGGTAGAGTACTCGGCTT 40.9 50.0 57.9 58.2 796-800 bp 1.0 62.4
matK-trnK-UUU_p2 TCAAGTTATGTTGCCAAGACCT ACGGTAGAGTACTCGGCTTT 40.9 50.0 57.9 58.2 795-799 bp 1.0 62.4
matK-trnK-UUU_p3 TCAAGTTATGTTGCCAAGACCT GGGTTGCTAACTCAACGGTA 40.9 50.0 57.9 57.5 809-813 bp 1.0 60.0
matK-trnK-UUU_p4 TCAAGTTATGTTGCCAAGACCT TTCGGGTTGCTAACTCAACG 40.9 50.0 57.9 58.5 812-816 bp 1.0 59.7
matK-trnK-UUU_p5 TCAAGTTATGTTGCCAAGACC ACGGTAGAGTACTCGGCTTT 42.9 50.0 56.4 58.2 795-799 bp 1.0 58.7