Hotspot

rpl22

LSC · 86902-87537 · 636 bp

Back to Xylocarpus

Pi 0.00786

MarkerSeek score 63.30

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 636
variable_sites: 5
pi: 0.00786
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CAAGTCGGAAACCAAGTGGA	GGAGAATTTGCGCCCACTAT	50.0	50.0	58.0	58.3	774 bp	1.0	77.7
rpl22_p2	ACCAAGTCGGAAACCAAGTG	GGAGAATTTGCGCCCACTAT	50.0	50.0	58.3	58.3	776 bp	1.0	75.4
rpl22_p3	CAAGTCGGAAACCAAGTGGA	GCGCCCACTATAAATTTCCG	50.0	50.0	58.0	57.3	765 bp	1.0	74.8
rpl22_p4	CAAGTCGGAAACCAAGTGGA	TCATTGGTGAGAGGTGAACC	50.0	50.0	58.0	57.1	685 bp	1.0	73.4
rpl22_p5	ACCAAGTCGGAAACCAAGTG	GCGCCCACTATAAATTTCCG	50.0	50.0	58.3	57.3	767 bp	1.0	72.5