Hotspot

trnL-CAA-ycf2

IRa · 148721-149729 · 1009 bp

Back to Xylocarpus

Pi 0.00496

MarkerSeek score 61.60

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: IRa
length_bp: 1009
variable_sites: 5
pi: 0.00496
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	AAATGGTAGACACGCGAGAC	ATGGCTTTTCCCGGATGAAA	50.0	45.0	58.0	57.8	1118 bp	1.0	78.5
trnL-CAA-ycf2_p2	AAATGGTAGACACGCGAGAC	TGGCTTTTCCCGGATGAAAT	50.0	45.0	58.0	57.8	1117 bp	1.0	78.5
trnL-CAA-ycf2_p3	TGAAATGGTAGACACGCGAG	ATGGCTTTTCCCGGATGAAA	50.0	45.0	58.0	57.8	1120 bp	1.0	78.4
trnL-CAA-ycf2_p4	TGAAATGGTAGACACGCGAG	TGGCTTTTCCCGGATGAAAT	50.0	45.0	58.0	57.8	1119 bp	1.0	78.4
trnL-CAA-ycf2_p5	AAATGGTAGACACGCGAGAC	AAGATGGCTTTTCCCGGATG	50.0	50.0	58.0	58.2	1121 bp	1.0	78.4