Hotspot

ycf2-trnL-CAA

IRb · 96912-97920 · 1009 bp

Back to Xylocarpus

Pi 0.00496

MarkerSeek score 61.60

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: IRb
length_bp: 1009
variable_sites: 5
pi: 0.00496
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	ATGGCTTTTCCCGGATGAAA	AAATGGTAGACACGCGAGAC	45.0	50.0	57.8	58.0	1118 bp	1.0	78.5
ycf2-trnL-CAA_p2	TGGCTTTTCCCGGATGAAAT	AAATGGTAGACACGCGAGAC	45.0	50.0	57.8	58.0	1117 bp	1.0	78.5
ycf2-trnL-CAA_p3	ATGGCTTTTCCCGGATGAAA	TGAAATGGTAGACACGCGAG	45.0	50.0	57.8	58.0	1120 bp	1.0	78.4
ycf2-trnL-CAA_p4	TGGCTTTTCCCGGATGAAAT	TGAAATGGTAGACACGCGAG	45.0	50.0	57.8	58.0	1119 bp	1.0	78.4
ycf2-trnL-CAA_p5	AAGATGGCTTTTCCCGGATG	AAATGGTAGACACGCGAGAC	50.0	50.0	58.2	58.0	1121 bp	1.0	78.4