Hotspot

trnT-UGU-trnL-UAA

LSC · 49120-50100 · 981 bp

Back to Xylocarpus

Pi 0.00306
MarkerSeek score 60.70
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
981
variable_sites
3
pi
0.00306
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 1029-1031 bp 1.0 79.3
trnT-UGU-trnL-UAA_p2 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 1037-1039 bp 1.0 78.8
trnT-UGU-trnL-UAA_p3 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 50.0 50.0 57.8 58.0 1061-1063 bp 1.0 78.4
trnT-UGU-trnL-UAA_p4 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 50.0 50.0 57.8 58.1 1069-1071 bp 1.0 77.9
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 1043-1045 bp 1.0 77.4