Hotspot

psbM-trnD-GUC

LSC · 31350-31919 · 570 bp

Back to Xylocarpus

Pi 0.00183

MarkerSeek score 63.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 570
variable_sites: 1
pi: 0.00183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TGAATCACTTTGACTGACCGT	TTCAATTGGTCAGAGCACCG	42.9	50.0	57.5	58.5	716-739 bp	1.0	67.1
psbM-trnD-GUC_p2	TGAATCACTTTGACTGACCGT	CCGGGATTGGGATTGTAGTT	42.9	50.0	57.5	57.3	734-757 bp	1.0	65.0
psbM-trnD-GUC_p3	TGAATCACTTTGACTGACCGT	GTTCAATTGGTCAGAGCACC	42.9	50.0	57.5	57.0	717-740 bp	1.0	62.9
psbM-trnD-GUC_p4	ATGAATCACTTTGACTGACCGT	TTCAATTGGTCAGAGCACCG	40.9	50.0	58.0	58.5	717-740 bp	1.0	62.8
psbM-trnD-GUC_p5	TGAATCACTTTGACTGACCGTT	TTCAATTGGTCAGAGCACCG	40.9	50.0	58.2	58.5	716-739 bp	1.0	61.3