Hotspot

petN-psbM

LSC · 30151-31235 · 1085 bp

Back to Xylocarpus

Pi 0.00185

MarkerSeek score 59.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1085
variable_sites: 2
pi: 0.00185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	CTTGGGCCGCTTTAATGGTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1244-1245 bp	1.0	67.0
petN-psbM_p2	TTGGGCCGCTTTAATGGTAG	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1243-1244 bp	1.0	67.0
petN-psbM_p3	CTTGGGCCGCTTTAATGGTA	ACGGTCAGTCAAAGTGATTCA	50.0	42.9	58.2	57.5	1186-1187 bp	1.0	66.5
petN-psbM_p4	TTGGGCCGCTTTAATGGTAG	ACGGTCAGTCAAAGTGATTCA	50.0	42.9	58.2	57.5	1185-1186 bp	1.0	66.5
petN-psbM_p5	GGCCGCTTTAATGGTAGTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.7	57.6	1240-1241 bp	1.0	66.3