Hotspot

trnC-GCA-petN

LSC · 29349-30060 · 712 bp

Back to Xylocarpus

Pi 0.00281

MarkerSeek score 60.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 2
pi: 0.00281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	CTACCATTAAAGCGGCCCAA	45.0	50.0	57.9	58.2	847 bp	1.0	77.8
trnC-GCA-petN_p2	TTTTGTATCGTTTTGGCGGC	TACCATTAAAGCGGCCCAAG	45.0	50.0	57.9	58.2	846 bp	1.0	77.8
trnC-GCA-petN_p3	TTTTGTATCGTTTTGGCGGC	AGACTACCATTAAAGCGGCC	45.0	50.0	57.9	57.7	850 bp	1.0	77.1
trnC-GCA-petN_p4	TTTTGTATCGTTTTGGCGGC	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.9	57.6	895 bp	1.0	76.9
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	TACCATTAAAGCGGCCCAAG	50.0	50.0	58.4	58.2	808 bp	1.0	75.4