Hotspot

trnK-UUU-rps16

LSC · 4470-5341 · 872 bp

Back to Xylocarpus

Pi 0.00232
MarkerSeek score 61.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
872
variable_sites
2
pi
0.00232
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGCCGTACTTAAAAGCCGAG GCGCTCAACCTACAGGAAC 50.0 57.9 58.0 58.5 997-1006 bp 1.0 68.6
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT GCGCTCAACCTACAGGAAC 50.0 57.9 58.2 58.5 986-995 bp 1.0 67.3
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT GCGCTCAACCTACAGGAAC 50.0 57.9 58.2 58.5 985-994 bp 1.0 67.3
trnK-UUU-rps16_p4 GCCGTACTTAAAAGCCGAGT GCGCTCAACCTACAGGAAC 50.0 57.9 58.3 58.5 996-1005 bp 1.0 66.4
trnK-UUU-rps16_p5 TACCGTTGAGTTAGCAACCC GCGCTCAACCTACAGGAAC 50.0 57.9 57.5 58.5 972-981 bp 1.0 65.0