Hotspot

rpl36

LSC · 83914-84027 · 114 bp

Back to Xylia

Pi 0.0439
MarkerSeek score 66.40
Primer available yes
Label rpl36

Feature Metadata

label_name
rpl36
feature_type
gene
region
LSC
length_bp
114
variable_sites
5
pi
0.0439
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36_p1 TTTTTGTGCATCCGGTCAGC AGATTCGGAATTCAGTTAAGGA 50.0 36.4 59.7 54.9 202-203 bp 1.0 42.4
rpl36_p2 TTTTTGTGCATCCGGTCAGC AGATTCGGAATTCAGTTAAGGAA 50.0 34.8 59.7 55.7 202-203 bp 1.0 42.4
rpl36_p3 TTTTGTGCATCCGGTCAGC AGATTCGGAATTCAGTTAAGGA 52.6 36.4 59.0 54.9 201-202 bp 1.0 42.4
rpl36_p4 TTTTTGTGCATCCGGTCAGC GATTCGGAATTCAGTTAAGGA 50.0 38.1 59.7 53.3 201-202 bp 1.0 42.4
rpl36_p5 TTTTTGTGCATCCGGTCAGC AGATTCGGAATTCAGTTAAGG 50.0 38.1 59.7 53.3 202-203 bp 1.0 42.4