Hotspot

rpl32

SSC · 133718-133885 · 168 bp

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Pi 0.0595
MarkerSeek score 68.30
Primer available yes
Label rpl32

Feature Metadata

label_name
rpl32
feature_type
gene
region
SSC
length_bp
168
variable_sites
10
pi
0.0595
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32_p1 GTCTTTCACATCCACCTATAGCA AGCGTGTCTACCGATTTCAC 43.5 50.0 58.0 58.0 661-732 bp 1.0 63.0
rpl32_p2 TGTCTTTCACATCCACCTATAGC AGCGTGTCTACCGATTTCAC 43.5 50.0 58.0 58.0 662-733 bp 1.0 63.0
rpl32_p3 GTCTTTCACATCCACCTATAGCA CAGCGTGTCTACCGATTTCA 43.5 50.0 58.0 58.0 662-733 bp 1.0 62.9
rpl32_p4 TGTCTTTCACATCCACCTATAGC CAGCGTGTCTACCGATTTCA 43.5 50.0 58.0 58.0 663-734 bp 1.0 62.9
rpl32_p5 AGCAGGATAAAGATAAGGGTTGG AGCGTGTCTACCGATTTCAC 43.5 50.0 57.9 58.0 951-1013 bp 1.0 62.4