Hotspot

clpP

LSC · 75164-75853 · 690 bp

Pi 0.244

MarkerSeek score 80.40

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 166
pi: 0.244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ACCCCGTTTTCCAATTTCGA	CAGAACGAGAACGGGCTTAA	45.0	50.0	58.0	57.9	981 bp	0.5	59.3
clpP_p2	ACCCCGTTTTCCAATTTCGA	TAAGAATGCGCAATGGTGGA	45.0	45.0	58.0	57.9	1368 bp	0.5	58.9
clpP_p3	ACCCCGTTTTCCAATTTCGA	GTTGTTCATCACTCAGGGGG	45.0	55.0	58.0	58.2	1298 bp	0.5	58.5
clpP_p4	ACCCCGTTTTCCAATTTCGA	GTAAGAATGCGCAATGGTGG	45.0	50.0	58.0	57.8	1369 bp	0.5	58.3
clpP_p5	ACCCCGTTTTCCAATTTCGA	ACCAAGGCAAACCCATGAAA	45.0	45.0	58.0	58.2	1577 bp	0.5	58.3