Hotspot

rpl32-trnL-UAG

IRa · 133886-134372 · 487 bp

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Pi 0.0409
MarkerSeek score 66.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
IRa
length_bp
487
variable_sites
17
pi
0.0409
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.85
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CCATTTGGAAGGAGAAGGGG AGCGTGTCTACCGATTTCAC 55.0 50.0 57.9 58.0 632 bp 0.5 58.7
rpl32-trnL-UAG_p2 CCATTTGGAAGGAGAAGGGG CAGCGTGTCTACCGATTTCA 55.0 50.0 57.9 58.0 633 bp 0.5 58.7
rpl32-trnL-UAG_p3 CCATTTGGAAGGAGAAGGGG TATCATGCCCTTTTCGGACG 55.0 50.0 57.9 58.1 919 bp 0.5 58.4
rpl32-trnL-UAG_p4 CCATTTGGAAGGAGAAGGGG GCTGCATAGCCCAATACCAT 55.0 50.0 57.9 58.1 1233 bp 0.5 58.1
rpl32-trnL-UAG_p5 CCATTTGGAAGGAGAAGGGG GAAGTAGCAAAGCCTTGGGT 55.0 50.0 57.9 58.1 1131 bp 0.5 58.1