Hotspot

ndhI-ndhG

IRb · 124775-125393 · 619 bp

Pi 0.0217

MarkerSeek score 74.10

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: IRb
length_bp: 619
variable_sites: 13
pi: 0.0217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	AATCAAATTGCTTTGGGCCG	CAAGACCCGATCCCAGAAAG	45.0	55.0	57.9	58.0	733-752 bp	1.0	82.4
ndhI-ndhG_p2	AATCAAATTGCTTTGGGCCG	AAGACCCGATCCCAGAAAGA	45.0	50.0	57.9	58.0	732-751 bp	1.0	82.3
ndhI-ndhG_p3	AATCAAATTGCTTTGGGCCG	TCCCAACGAAAAGGCAGAAA	45.0	45.0	57.9	57.9	795-814 bp	1.0	81.9
ndhI-ndhG_p4	AATCAAATTGCTTTGGGCCG	AGACCCGATCCCAGAAAGAT	45.0	50.0	57.9	57.8	731-750 bp	1.0	81.2
ndhI-ndhG_p5	TTTGGGCCGTTTACCAATGT	CAAGACCCGATCCCAGAAAG	45.0	55.0	58.3	58.0	722-741 bp	1.0	80.9