Hotspot

clpP-psbB

LSC · 75854-76586 · 733 bp

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Pi 0.137
MarkerSeek score 81.80
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
733
variable_sites
98
pi
0.137
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 GGTCGGATCCTCCATACTGA ATCAAAACCTTCCACACCCC 55.0 50.0 58.0 58.0 1486 bp 0.5 59.9
clpP-psbB_p2 GCCAGCAAGTTGAGTCGATA ATCAAAACCTTCCACACCCC 50.0 50.0 58.0 58.0 1453 bp 0.5 59.8
clpP-psbB_p3 GGTCGGATCCTCCATACTGA ACACCCATATTCCAGGACCA 55.0 50.0 58.0 58.0 1418 bp 0.5 59.8
clpP-psbB_p4 GGTCGGATCCTCCATACTGA ATACTCCAACCACCCCATGA 55.0 50.0 58.0 58.0 1152 bp 0.5 59.8
clpP-psbB_p5 GGTCGGATCCTCCATACTGA TGATACTCCAACCACCCCAT 55.0 50.0 58.0 58.0 1154 bp 0.5 59.8