Hotspot

psbE-psbF

LSC · 73731-73739 · 9 bp

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Pi 0.111
MarkerSeek score 77.00
Primer available yes
Label psbE-psbF

Feature Metadata

label_name
psbE-psbF
feature_type
igs
region
LSC
length_bp
9
variable_sites
1
pi
0.111
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-psbF_p1 AACTGGCCGTTTTGATCCTT GAACAGCCAACCATCGTACT 45.0 50.0 58.0 57.9 116 bp 1.0 79.9
psbE-psbF_p2 ACTGGCCGTTTTGATCCTTT GAACAGCCAACCATCGTACT 45.0 50.0 58.0 57.9 115 bp 1.0 79.9
psbE-psbF_p3 AACTGGCCGTTTTGATCCTT AACAGCCAACCATCGTACTG 45.0 50.0 58.0 58.2 115 bp 1.0 79.1
psbE-psbF_p4 ACTGGCCGTTTTGATCCTTT AACAGCCAACCATCGTACTG 45.0 50.0 58.0 58.2 114 bp 1.0 79.1
psbE-psbF_p5 ACTGGCCGTTTTGATCCTTT TGAACAGCCAACCATCGTAC 45.0 50.0 58.0 58.2 116 bp 1.0 79.1