Hotspot

accD-psaI

LSC · 63193-63781 · 589 bp

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Pi 0.0348
MarkerSeek score 68.40
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
589
variable_sites
17
pi
0.0348
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.83
markerseek_score
68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 GCTCCATGCTTTCTTTCCTT CCATTGCAATTGCCGGAAAT 45.0 45.0 56.3 58.0 597-696 bp 1.0 72.3
accD-psaI_p2 GCTCCATGCTTTCTTTCCTT AAGAAGCCATTGCAATTGCC 45.0 45.0 56.3 57.9 603-702 bp 1.0 71.6
accD-psaI_p3 GCTCCATGCTTTCTTTCCTTT CCATTGCAATTGCCGGAAAT 42.9 45.0 57.1 58.0 597-696 bp 1.0 70.3
accD-psaI_p4 GCTCCATGCTTTCTTTCCTTT AAGAAGCCATTGCAATTGCC 42.9 45.0 57.1 57.9 603-702 bp 1.0 69.7
accD-psaI_p5 GTGAGTTATTTCAGCTCCATGC CCATTGCAATTGCCGGAAAT 45.5 45.0 57.7 58.0 610-709 bp 1.0 67.6