Hotspot

ndhC-trnV-UAC

LSC · 54726-55350 · 625 bp

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Pi 0.0175
MarkerSeek score 69.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
625
variable_sites
9
pi
0.0175
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.82
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTGGCCCTTTACTAATCGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 57.7 57.8 668-773 bp 1.0 81.4
ndhC-trnV-UAC_p2 CTGGCCCTTTACTAATCGGG AAATGCACGTTGGGTCTGTA 55.0 45.0 57.7 57.7 736-841 bp 1.0 80.8
ndhC-trnV-UAC_p3 CTGGCCCTTTACTAATCGGG AATGCACGTTGGGTCTGTAA 55.0 45.0 57.7 57.7 735-840 bp 1.0 80.8
ndhC-trnV-UAC_p4 CTGGCCCTTTACTAATCGGG ATGCACGTTGGGTCTGTAAA 55.0 45.0 57.7 57.7 734-839 bp 1.0 80.8
ndhC-trnV-UAC_p5 CTGGCCCTTTACTAATCGGG ACGTTGGGTCTGTAAAGCAA 55.0 45.0 57.7 57.7 730-835 bp 1.0 80.2