Hotspot

rps16

LSC · 4765-5518 · 754 bp

Back to Xerophyta

Pi 0.0252

MarkerSeek score 69.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 754
variable_sites: 63
pi: 0.0252
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGACCTCGGCAAAACATTGA	TGTGATTGCGTCGATCTGTT	45.0	45.0	58.0	57.9	1937-2306 bp	1.0	89.5
rps16_p2	TGACCTCGGCAAAACATTGA	TTGTGATTGCGTCGATCTGT	45.0	45.0	58.0	57.9	1938-2307 bp	1.0	89.5
rps16_p3	TGACCTCGGCAAAACATTGA	GTTTGGGAAAACTGGGACCT	45.0	50.0	58.0	57.9	1701-2089 bp	0.928571	85.4
rps16_p4	GCTTGTTGAGGATTCCCACA	TGTGATTGCGTCGATCTGTT	50.0	45.0	58.1	57.9	1442-1802 bp	0.714286	75.9
rps16_p5	GCTTGTTGAGGATTCCCACA	TTGTGATTGCGTCGATCTGT	50.0	45.0	58.1	57.9	1443-1803 bp	0.714286	75.9