Hotspot

ndhE-ndhG

SSC · 118388-118565 · 178 bp

Back to Xerophyta

Pi 0.0351

MarkerSeek score 67.90

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 178
variable_sites: 19
pi: 0.0351
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	ACATCATTGACAAACCCCTCA	ATTACTATGGCTCGTCAATAA	42.9	33.3	57.5	52.2	189-221 bp	1.0	51.3
ndhE-ndhG_p2	CGAACATCATTGACAAACCCC	ATTACTATGGCTCGTCAATAA	47.6	33.3	57.8	52.2	192-224 bp	1.0	51.2
ndhE-ndhG_p3	TGCTCTAACCATGTTTCGACT	ATTACTATGGCTCGTCAATAA	42.9	33.3	57.3	52.2	272-304 bp	1.0	49.5
ndhE-ndhG_p4	GTGCTCTAACCATGTTTCGAC	ATTACTATGGCTCGTCAATAA	47.6	33.3	57.3	52.2	273-305 bp	1.0	49.5
ndhE-ndhG_p5	AGTGCTCTAACCATGTTTCGA	ATTACTATGGCTCGTCAATAA	42.9	33.3	57.3	52.2	274-306 bp	1.0	49.4