Hotspot

ndhF-rpl32

SSC · 113131-114036 · 906 bp

Back to Xerophyta

Pi 0.0323

MarkerSeek score 77.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 906
variable_sites: 97
pi: 0.0323
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGTCGGAACAAGCAGAAGT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.1	57.9	1542-1653 bp	1.0	88.7
ndhF-rpl32_p2	TGTCGGAACAAGCAGAAGTC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.1	57.9	1541-1652 bp	1.0	88.7
ndhF-rpl32_p3	CTGTCGGAACAAGCAGAAGT	AATTTCACCATGGCGGCTTA	50.0	45.0	58.1	58.2	1520-1631 bp	1.0	88.6
ndhF-rpl32_p4	ACTTGGTCGTGTGAACCAAA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.9	57.9	1958-2069 bp	1.0	88.1
ndhF-rpl32_p5	ACTTGGTCGTGTGAACCAAA	AATTTCACCATGGCGGCTTA	45.0	45.0	57.9	58.2	1936-2047 bp	1.0	88.0