Hotspot

rbcL-accD

LSC · 56104-56844 · 741 bp

Back to Xerophyta

Pi 0.0287

MarkerSeek score 70.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 741
variable_sites: 63
pi: 0.0287
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	ACTACAGTTCGGTGGAGGAA	GCCCACACCTATGTTCTAGC	50.0	55.0	58.0	58.0	1028-1091 bp	1.0	91.5
rbcL-accD_p2	ACTACAGTTCGGTGGAGGAA	ACTTCCACAGGTATGTCCCA	50.0	50.0	58.0	58.0	1078-1141 bp	1.0	91.2
rbcL-accD_p3	ACTACAGTTCGGTGGAGGAA	TAACTGCCACTATGGGTCCA	50.0	50.0	58.0	58.0	1140-1203 bp	1.0	91.1
rbcL-accD_p4	ACTACAGTTCGGTGGAGGAA	TGCCACTATGGGTCCAACTA	50.0	50.0	58.0	58.0	1136-1199 bp	1.0	91.1
rbcL-accD_p5	GAAGGGGAACGTGAGATGAC	ACTTCCACAGGTATGTCCCA	55.0	50.0	58.0	58.0	1269-1332 bp	0.857143	84.6