Hotspot

psbC-trnS-UGA

LSC · 34578-34726 · 149 bp

Back to Xerophyta

Pi 0.0485

MarkerSeek score 78.60

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 149
variable_sites: 18
pi: 0.0485
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	58.2	58.2	201-225 bp	1.0	88.0
psbC-trnS-UGA_p2	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.2	58.1	251-275 bp	1.0	87.9
psbC-trnS-UGA_p3	CGGCAGGATTTGAGAAAGGA	GGTTGATAGCTCCGGTCTTG	50.0	55.0	57.9	58.1	289-313 bp	1.0	87.6
psbC-trnS-UGA_p4	CGGCAGGATTTGAGAAAGGA	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.9	58.2	239-263 bp	1.0	87.3
psbC-trnS-UGA_p5	TTCCATGACCCCTCTTAGCT	GGTTGATAGCTCCGGTCTTG	50.0	55.0	57.7	58.1	243-267 bp	1.0	87.2