Hotspot

trnK-UUU-rps16

LSC · 4040-4764 · 725 bp

Back to Xerophyta

Pi 0.0342

MarkerSeek score 74.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 725
variable_sites: 73
pi: 0.0342
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TACCGTTGAGTTAGCAACCC	ATTTAAAGAAAGAACTTCGAGTT	50.0	26.1	57.5	52.6	745-818 bp	1.0	51.6
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	ATTTAAAGAAAGAACTTCGAGTT	50.0	26.1	58.2	52.6	759-832 bp	1.0	51.5
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	ATTTAAAGAAAGAACTTCGAGTT	50.0	26.1	58.2	52.6	758-831 bp	1.0	51.5
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	TTAAAGAAAGAACTTCGAGTTAA	50.0	26.1	58.2	52.1	756-829 bp	1.0	51.5
trnK-UUU-rps16_p5	AGTCGCACTTAAAAGCCGAG	ATTTAAAGAAAGAACTTCGAGTT	50.0	26.1	58.6	52.6	770-843 bp	1.0	51.4