Hotspot

ycf1

SSC · 107923-113127 · 5205 bp

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Pi 0.0195

MarkerSeek score 55.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5205
variable_sites: 150
pi: 0.0195
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGGGACCGGCTCAAATAAAT	TGTTTATGTGGAATGTTCTCCCA	50.0	39.1	57.9	58.0	214 bp	1.0	62.1
ycf1_p2	GGGGACCGGCTCAAATAAAT	GTTTATGTGGAATGTTCTCCCA	50.0	40.9	57.9	56.3	213 bp	1.0	56.9
ycf1_p3	GGGGACCGGCTCAAATAAAT	TGTTTATGTGGAATGTTCTCCC	50.0	40.9	57.9	56.3	214 bp	1.0	56.8
ycf1_p4	ACCGGCTCAAATAAATCCCA	TGTTTATGTGGAATGTTCTCCCA	45.0	39.1	57.2	58.0	210 bp	1.0	56.2
ycf1_p5	ATCTATGCGCACTCAAAGGC	TGTTTATGTGGAATGTTCTCCCA	50.0	39.1	58.7	58.0	249 bp	1.0	56.1