Hotspot

accD

LSC · 56486-57949 · 1464 bp

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Pi 0.0114
MarkerSeek score 51.70
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
1464
variable_sites
25
pi
0.0114
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 AGGCTACCAAATGGAGTCCT CTAAAGGCACCAAAACAGCG 50.0 50.0 58.0 57.9 2800-2826 bp 1.0 81.0
accD_p2 GCCGTAGCTAATCGAGTAGC CTAAAGGCACCAAAACAGCG 55.0 50.0 58.0 57.9 2891-2917 bp 1.0 80.9
accD_p3 GGCTACCAAATGGAGTCCTG CTAAAGGCACCAAAACAGCG 55.0 50.0 58.0 57.9 2799-2825 bp 1.0 80.9
accD_p4 AGGCTACCAAATGGAGTCCT AAAGAAGCCATTGCCATTGC 50.0 45.0 58.0 57.9 2837-2863 bp 1.0 80.6
accD_p5 AGGCTACCAAATGGAGTCCT GGGACGGCTTTTATGTGACT 50.0 50.0 58.0 57.9 2250-2262 bp 1.0 80.5