Hotspot

ndhD-ccsA

SSC · 121383-121633 · 251 bp

Back to Xeranthemum

Pi 0.0239
MarkerSeek score 64.10
Primer available yes
Label ndhD-ccsA

Feature Metadata

label_name
ndhD-ccsA
feature_type
igs
region
SSC
length_bp
251
variable_sites
9
pi
0.0239
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-ccsA_p1 GGTATTGGCATGTATCCCGA TGGATATGCTATTTTGGGGTCA 50.0 40.9 57.1 57.2 413-431 bp 1.0 54.6
ndhD-ccsA_p2 AGGTATTGGCATGTATCCCG TGGATATGCTATTTTGGGGTCA 50.0 40.9 57.1 57.2 414-432 bp 1.0 54.5
ndhD-ccsA_p3 TGGCATGTATCCCGATTTTGT TGGATATGCTATTTTGGGGTCA 42.9 40.9 58.0 57.2 408-426 bp 1.0 53.3
ndhD-ccsA_p4 GGTATTGGCATGTATCCCGA TGGATATGCTATTTTGGGGTCAA 50.0 39.1 57.1 57.9 413-431 bp 1.0 51.9
ndhD-ccsA_p5 GGTATTGGCATGTATCCCGA TTGGATATGCTATTTTGGGGTCA 50.0 39.1 57.1 57.9 414-432 bp 1.0 51.9