Hotspot

psaC-ndhD

SSC · 119750-119879 · 130 bp

Back to Xeranthemum

Pi 0.0267

MarkerSeek score 66.70

Primer available yes

Label psaC-ndhD

Feature Metadata

label_name: psaC-ndhD
feature_type: igs
region: SSC
length_bp: 130
variable_sites: 5
pi: 0.0267
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhD_p1	ACAGAGGATTGTGTCGGTTG	GGAACCCGCAAAGATTGGTA	50.0	50.0	57.8	58.2	295-301 bp	1.0	80.2
psaC-ndhD_p2	CAGAGGATTGTGTCGGTTGT	GGAACCCGCAAAGATTGGTA	50.0	50.0	57.8	58.2	294-300 bp	1.0	80.2
psaC-ndhD_p3	AAGAGATGTGAATCCGCCTG	GGAACCCGCAAAGATTGGTA	50.0	50.0	57.7	58.2	274-280 bp	1.0	79.2
psaC-ndhD_p4	GCCCAACGGATTTCTTGAGT	GGAACCCGCAAAGATTGGTA	50.0	50.0	58.5	58.2	255-261 bp	1.0	78.3
psaC-ndhD_p5	GAACAGAGGATTGTGTCGGT	GGAACCCGCAAAGATTGGTA	50.0	50.0	57.5	58.2	297-303 bp	1.0	77.9