Pi
0.0375
MarkerSeek score
79.50
Primer available
yes
Label
rpl16
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rpl16_p1 | CTGCTCTTTCAATGCAAAGGA | TCCTCCTGTTTGTTGAATCA | 42.9 | 40.0 | 57.1 | 54.5 | 2160-2376 bp | 1.0 | 45.5 |
| rpl16_p2 | TCTGCTCTTTCAATGCAAAGG | TCCTCCTGTTTGTTGAATCA | 42.9 | 40.0 | 57.1 | 54.5 | 2161-2377 bp | 1.0 | 45.5 |
| rpl16_p3 | CTGCTCTTTCAATGCAAAGGA | TCCTCCTGTTTGTTGAATCAA | 42.9 | 38.1 | 57.1 | 55.3 | 2160-2376 bp | 1.0 | 45.5 |
| rpl16_p4 | CTGCTCTTTCAATGCAAAGGA | TTCCTCCTGTTTGTTGAATCA | 42.9 | 38.1 | 57.1 | 55.3 | 2161-2377 bp | 1.0 | 45.5 |
| rpl16_p5 | TCTGCTCTTTCAATGCAAAGG | TCCTCCTGTTTGTTGAATCAA | 42.9 | 38.1 | 57.1 | 55.3 | 2161-2377 bp | 1.0 | 45.5 |