Hotspot

psaC-ndhE

SSC · 125259-125888 · 630 bp

Back to Xanthosoma

Pi 0.0295

MarkerSeek score 78.50

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 630
variable_sites: 18
pi: 0.0295
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	AGCTTTACATCCTGGCCAAG	GCTGAAGCTGCTATTGGTCT	50.0	50.0	57.9	58.0	831-1084 bp	1.0	81.7
psaC-ndhE_p2	GGCCCGTACACATTGAGTAC	GCTGAAGCTGCTATTGGTCT	55.0	50.0	58.4	58.0	783-1036 bp	1.0	80.2
psaC-ndhE_p3	GCCCGTACACATTGAGTACA	GCTGAAGCTGCTATTGGTCT	50.0	50.0	57.6	58.0	782-1035 bp	1.0	80.0
psaC-ndhE_p4	AGGCCCGTACACATTGAGTA	GCTGAAGCTGCTATTGGTCT	50.0	50.0	58.4	58.0	784-1037 bp	1.0	79.5
psaC-ndhE_p5	CTGGCCAAGGTATCATTTCCA	GCTGAAGCTGCTATTGGTCT	47.6	50.0	58.0	58.0	820-1073 bp	1.0	74.7