Hotspot

ndhF-rpl32

SSC · 119933-121244 · 1312 bp

Back to Xanthosoma

Pi 0.00783
MarkerSeek score 65.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1312
variable_sites
10
pi
0.00783
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACAGGGAGTGGAAGAAAAGG CGCAGCCAAATATCCTTTTT 50.0 40.0 57.0 54.9 1449-1462 bp 1.0 50.1
ndhF-rpl32_p2 CAGGGAGTGGAAGAAAAGGT CGCAGCCAAATATCCTTTTT 50.0 40.0 57.0 54.9 1448-1461 bp 1.0 50.1
ndhF-rpl32_p3 AACAGGGAGTGGAAGAAAAGG CGCAGCCAAATATCCTTTTT 47.6 40.0 57.8 54.9 1450-1463 bp 1.0 47.9
ndhF-rpl32_p4 ACAGGGAGTGGAAGAAAAGGT CGCAGCCAAATATCCTTTTT 47.6 40.0 58.9 54.9 1449-1462 bp 1.0 42.8
ndhF-rpl32_p5 TGACATAGTAACAGGGAGTGGA CGCAGCCAAATATCCTTTTT 45.5 40.0 57.9 54.9 1459-1472 bp 1.0 42.5