Hotspot

trnF(GAA)-ndhJ

LSC · 52040-53428 · 1389 bp

Back to Xanthosoma

Pi 0.0618

MarkerSeek score 83.20

Primer available yes

Label trnF(GAA)-ndhJ

Feature Metadata

label_name: trnF(GAA)-ndhJ
feature_type: igs
region: LSC
length_bp: 1389
variable_sites: 82
pi: 0.0618
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF(GAA)-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	1499-1706 bp	1.0	85.7
trnF(GAA)-ndhJ_p2	AATCCTCGTGTCACCAGTTC	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.5	57.9	1502-1709 bp	1.0	82.5
trnF(GAA)-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGCTGGCC	50.0	55.0	58.1	58.6	1498-1705 bp	1.0	81.9
trnF(GAA)-ndhJ_p4	TGGTAGAGCAGAGGACTGAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.4	57.9	1522-1729 bp	1.0	81.2
trnF(GAA)-ndhJ_p5	TTGGTAGAGCAGAGGACTGA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.4	57.9	1523-1730 bp	1.0	81.2