Hotspot

ycf3-trnS(GGA)

LSC · 47617-48493 · 877 bp

Back to Xanthosoma

Pi 0.0115
MarkerSeek score 65.80
Primer available yes
Label ycf3-trnS(GGA)

Feature Metadata

label_name
ycf3-trnS(GGA)
feature_type
igs
region
LSC
length_bp
877
variable_sites
10
pi
0.0115
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS(GGA)_p1 TTCTCCTGAAGTTGTCGGAA CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.4 58.1 1040-1054 bp 1.0 67.6
ycf3-trnS(GGA)_p2 TTTCTCCTGAAGTTGTCGGA CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.4 58.1 1041-1055 bp 1.0 67.6
ycf3-trnS(GGA)_p3 TTCTCCTGAAGTTGTCGGAA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 58.2 1061-1075 bp 1.0 67.2
ycf3-trnS(GGA)_p4 TTTCTCCTGAAGTTGTCGGA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 58.2 1062-1076 bp 1.0 67.2
ycf3-trnS(GGA)_p5 TTCTCCTGAAGTTGTCGGAA TTCCAATGCTACGCCTTGAA 45.0 45.0 56.4 57.8 1011-1025 bp 1.0 66.9