Hotspot

trnY(GUA)-trnE(UUC)

LSC · 33585-33879 · 295 bp

Back to Xanthosoma

Pi 0.111

MarkerSeek score 80.80

Primer available yes

Label trnY(GUA)-trnE(UUC)

Feature Metadata

label_name: trnY(GUA)-trnE(UUC)
feature_type: igs
region: LSC
length_bp: 295
variable_sites: 32
pi: 0.111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY(GUA)-trnE(UUC)_p1	CACAAAAGAAAGCGGCGAAT	TAGCGGTTCAGGACATCTCT	45.0	50.0	57.9	57.9	463-552 bp	1.0	84.7
trnY(GUA)-trnE(UUC)_p2	CACAAAAGAAAGCGGCGAAT	TCTAGCGGTTCAGGACATCT	45.0	50.0	57.9	57.9	465-554 bp	1.0	84.7
trnY(GUA)-trnE(UUC)_p3	AGAAAGCGGCGAATTTTTGG	TAGCGGTTCAGGACATCTCT	45.0	50.0	57.9	57.9	457-546 bp	1.0	84.7
trnY(GUA)-trnE(UUC)_p4	AGAAAGCGGCGAATTTTTGG	TCTAGCGGTTCAGGACATCT	45.0	50.0	57.9	57.9	459-548 bp	1.0	84.7
trnY(GUA)-trnE(UUC)_p5	CACAAAAGAAAGCGGCGAAT	GTCTAGCGGTTCAGGACATC	45.0	55.0	57.9	57.8	466-555 bp	1.0	84.2