Hotspot

trnS(GCU)-trnS (CGA)

LSC · 8861-10658 · 1798 bp

Back to Xanthosoma

Pi 0.0713

MarkerSeek score 86.50

Primer available yes

Label trnS(GCU)-trnS (CGA)

Feature Metadata

label_name: trnS(GCU)-trnS (CGA)
feature_type: igs
region: LSC
length_bp: 1798
variable_sites: 121
pi: 0.0713
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 86.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(GCU)-trnS (CGA)_p1	CAATCCGTCGCTTTAGTCCA	AGGACGAATCACACTTTTACCA	50.0	40.9	58.0	57.7	1870-2098 bp	1.0	70.1
trnS(GCU)-trnS (CGA)_p2	AATCCGTCGCTTTAGTCCAC	AGGACGAATCACACTTTTACCA	50.0	40.9	58.0	57.7	1869-2097 bp	1.0	70.1
trnS(GCU)-trnS (CGA)_p3	GAGAAAGCGGAGAAAGAGGG	AGGACGAATCACACTTTTACCA	55.0	40.9	58.0	57.7	1930-2158 bp	1.0	69.8
trnS(GCU)-trnS (CGA)_p4	CAATCCGTCGCTTTAGTCCA	GGACGAATCACACTTTTACCAC	50.0	45.5	58.0	57.6	1869-2097 bp	1.0	69.6
trnS(GCU)-trnS (CGA)_p5	AATCCGTCGCTTTAGTCCAC	GGACGAATCACACTTTTACCAC	50.0	45.5	58.0	57.6	1868-2096 bp	1.0	69.6