Hotspot

psaC

SSC · 120127-120372 · 246 bp

Back to Xanthium

Pi 0.00407

MarkerSeek score 40.80

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 1
pi: 0.00407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	AAAACTCGGGGAACTCATCG	TCTTTCCTCCCCACATGGAT	50.0	50.0	57.9	58.0	947-998 bp	1.0	80.4
psaC_p2	CTCGGGGAACTCATCGATTC	TCTTTCCTCCCCACATGGAT	55.0	50.0	57.9	58.0	943-994 bp	1.0	80.1
psaC_p3	AAAACTCGGGGAACTCATCG	GAGACGTTTCTTTCCTCCCC	50.0	55.0	57.9	57.9	955-1006 bp	1.0	79.8
psaC_p4	TGGACTCGCCATTGTTTCAT	TCTTTCCTCCCCACATGGAT	45.0	50.0	57.8	58.0	1156-1209 bp	1.0	79.6
psaC_p5	AAAACTCGGGGAACTCATCG	TTCCAAAGCCACAGGATACG	50.0	50.0	57.9	57.9	1102-1153 bp	1.0	79.5