Hotspot

ndhF

SSC · 125064-127295 · 2232 bp

Back to Xanthium

Pi 0.00717

MarkerSeek score 47.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 16
pi: 0.00717
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AACGAGGTAAGATCCGGTGA	GCCGAGAAGAAGGTATCAGC	50.0	55.0	58.2	58.1	2737-2784 bp	1.0	78.3
ndhF_p2	ACGAGGTAAGATCCGGTGAA	GCCGAGAAGAAGGTATCAGC	50.0	55.0	58.2	58.1	2736-2783 bp	1.0	78.3
ndhF_p3	AACGAGGTAAGATCCGGTGA	CTAGCACTGGGTAGACCTCA	50.0	55.0	58.2	57.9	2653-2700 bp	1.0	78.3
ndhF_p4	ACGAGGTAAGATCCGGTGAA	CTAGCACTGGGTAGACCTCA	50.0	55.0	58.2	57.9	2652-2699 bp	1.0	78.3
ndhF_p5	AGACCGATGCCCTTAGTTCT	CTAGCACTGGGTAGACCTCA	50.0	55.0	58.1	57.9	2917 bp	0.5	57.8