Hotspot

trnR-rrn5

IRa · 128857-129137 · 281 bp

Back to Xanthium

Pi 0.0154
MarkerSeek score 65.40
Primer available yes
Label trnR-rrn5

Feature Metadata

label_name
trnR-rrn5
feature_type
igs
region
IRa
length_bp
281
variable_sites
4
pi
0.0154
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-rrn5_p1 CCACGTGCTCTAATCCTCTG GGTGACGATACTGTAGGGGA 55.0 55.0 57.8 57.9 345-365 bp 1.0 82.7
trnR-rrn5_p2 CCACGTGCTCTAATCCTCTG AATCCATCCCGAACTTGGTG 55.0 50.0 57.8 57.9 380-400 bp 1.0 82.1
trnR-rrn5_p3 CCACGTGCTCTAATCCTCTG ACCAATCCATCCCGAACTTG 55.0 50.0 57.8 57.9 383-403 bp 1.0 82.1
trnR-rrn5_p4 CCACGTGCTCTAATCCTCTG CAATCCATCCCGAACTTGGT 55.0 50.0 57.8 57.9 381-401 bp 1.0 82.1
trnR-rrn5_p5 CCACGTGCTCTAATCCTCTG CACCAATCCATCCCGAACTT 55.0 50.0 57.8 57.9 384-404 bp 1.0 82.1