Hotspot

psaC-ndhD

SSC · 120373-120463 · 91 bp

Back to Xanthium

Pi 0.112

MarkerSeek score 83.40

Primer available yes

Label psaC-ndhD

Feature Metadata

label_name: psaC-ndhD
feature_type: igs
region: SSC
length_bp: 91
variable_sites: 10
pi: 0.112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhD_p1	ACAGAGGATTGTGTCGGTTG	ACCAAGGAAATTTGTTCGTGG	50.0	42.9	57.8	57.3	251-302 bp	1.0	68.3
psaC-ndhD_p2	ACAGAGGATTGTGTCGGTTG	CCAAGGAAATTTGTTCGTGGT	50.0	42.9	57.8	57.3	250-301 bp	1.0	68.3
psaC-ndhD_p3	CAGAGGATTGTGTCGGTTGT	ACCAAGGAAATTTGTTCGTGG	50.0	42.9	57.8	57.3	250-301 bp	1.0	68.3
psaC-ndhD_p4	CAGAGGATTGTGTCGGTTGT	CCAAGGAAATTTGTTCGTGGT	50.0	42.9	57.8	57.3	249-300 bp	1.0	68.3
psaC-ndhD_p5	AAGAGATGTGAATCCGCCTG	CCAAGGAAATTTGTTCGTGGT	50.0	42.9	57.7	57.3	229-280 bp	1.0	67.4