Hotspot

rrn5-trnR

IRb · 107475-107755 · 281 bp

Back to Xanthium

Pi 0.0154
MarkerSeek score 65.40
Primer available yes
Label rrn5-trnR

Feature Metadata

label_name
rrn5-trnR
feature_type
igs
region
IRb
length_bp
281
variable_sites
4
pi
0.0154
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn5-trnR_p1 GGTGACGATACTGTAGGGGA CCACGTGCTCTAATCCTCTG 55.0 55.0 57.9 57.8 345-365 bp 1.0 82.7
rrn5-trnR_p2 AATCCATCCCGAACTTGGTG CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 380-400 bp 1.0 82.1
rrn5-trnR_p3 ACCAATCCATCCCGAACTTG CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 383-403 bp 1.0 82.1
rrn5-trnR_p4 CAATCCATCCCGAACTTGGT CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 381-401 bp 1.0 82.1
rrn5-trnR_p5 CACCAATCCATCCCGAACTT CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 384-404 bp 1.0 82.1