Hotspot

rpl16-rps3

LSC · 82663-82829 · 167 bp

Back to Xanthium

Pi 0.0323

MarkerSeek score 66.40

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 167
variable_sites: 5
pi: 0.0323
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AAACCAACGAGTCACACACT	ACAGTTCGAACAATCTATGGGG	45.0	45.5	57.6	58.1	244-256 bp	1.0	65.3
rpl16-rps3_p2	AAACCAACGAGTCACACACT	CAGTTCGAACAATCTATGGGGT	45.0	45.5	57.6	58.1	243-255 bp	1.0	65.3
rpl16-rps3_p3	AAACCAACGAGTCACACACT	AGTTCGAACAATCTATGGGGT	45.0	42.9	57.6	56.7	242-254 bp	1.0	64.0
rpl16-rps3_p4	AAACCAACGAGTCACACACT	CAGTTCGAACAATCTATGGGG	45.0	47.6	57.6	56.4	243-255 bp	1.0	61.6
rpl16-rps3_p5	AACGAGTCACACACTAAGCA	CAGTTCGAACAATCTATGGGGT	45.0	45.5	57.1	58.1	238-250 bp	1.0	61.5