Hotspot

clpP-psbB

LSC · 71973-72455 · 483 bp

Back to Xanthium

Pi 0.0128

MarkerSeek score 66.40

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 483
variable_sites: 6
pi: 0.0128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	TCCTCTCCAGGACTTCGAAA	CGATACCAAGGCAAACCCAT	50.0	50.0	57.7	58.2	537-550 bp	1.0	78.6
clpP-psbB_p2	TCCTCTCCAGGACTTCGAAA	AGCAACCAAAGCTGTATGCA	50.0	45.0	57.7	58.4	610-623 bp	1.0	77.1
clpP-psbB_p3	TCCTCTCCAGGACTTCGAAA	ACACGATACCAAGGCAAACC	50.0	50.0	57.7	58.5	540-553 bp	1.0	76.7
clpP-psbB_p4	GATGCATCTTCCTCTCCAGG	CGATACCAAGGCAAACCCAT	55.0	50.0	57.5	58.2	546-559 bp	1.0	76.6
clpP-psbB_p5	TCCTCTCCAGGACTTCGAAA	TGAACACGATACCAAGGCAA	50.0	45.0	57.7	57.4	543-556 bp	1.0	76.1